Predators

I checked Wikipedia and I found out that the first Predator film was actually released the same year I was born. About a month before I was born to be exact. Knowing that now is pretty weird because I grew up watching Predator movies I and II and is probably one of the first films that really got me to love movies. I think I was about 8 or 9 years old when I was able to watch the first one with Arnold (insert last name here) on cable. The second one with Danny Glover I didn't like as much but I still enjoyed.

Looking back now, I realize It was such a great suspense and adventure movie for me. Stranded in the jungle with an unknown creature killing you off one by one and in the end, you find yourself on your own and have to face this scary, dangerous extraterrestrial once and for all.

I just loved it. And now, 2010, we get to see a remake. Hmmm... hope they do this one right. Maybe I will like this new one too but we'll see :D Let's just enjoy the trailer for now.

Plants Vs. Zombies…

Hands down one of the most entertaining and challenging computer games I have ever played. I absolutely love it. I'm more of a RPG (role playing game) gamer and I'm not really into strategy games but I just cant help but get hooked with PVZ. I used to just play this in my friend's computer or at internet cafes to pass the time but now that we have it at home, I'm in gaming heaven :D

Just finished all the levels of the Adventure Mode. Not really a big fan of the Puzzle and Mini Games mode so I was really glad I finally unlocked the Survival mode when I finished the Adventure.

[caption id="" align="aligncenter" width="371" caption="Plants vs. Zombies"][/caption]

So far natapos ko na rin yung DAY level ng Survival. More Survival games to go. Hehehe.

No worries… I hope

I don't know if it was due to the stress of the day, my memory acting up on me, or I just had a bout of extreme stupidity :( It turns out that K.pneumoniae and E. cloacae do resemble each other in terms of colonial growth so there was really nothing to fuss about. Hehehe. I just had to make sure whether my bacteria is motile or non-motile because that is the only differential test between the two species I can do in our lab.

Here's a picture of the biochem tests I interpreted yesterday which I wasn't able to include in my last, hurried post:

[caption id="attachment_141" align="aligncenter" width="300" caption="from left: TSI, SIM, SCA, LIA"][/caption]

So anyways, when I got to school this morning I checked my SIM right away and after much scrutinizing, I realized it did become hazy as compared to the original clarity of an uninoculated SIM tube. I was focusing too much on the middle of the tube that I forgot to look at the over-all transparency of the medium.

So after yester-night's confusion, I was finally able to conclude that my final unknown bacteria in Clinical Bacteriology is indeed Enterobacter cloacae :D Whew... I just hope tama nga ako... toink!

Confusion!!!

Today I was able to interpret the results for the biochemical tests I did for my final unknown in Clinical Bacteriology lab. So far, from my last post, we already know that my unknown is a gram (-) bacilli, catalase (+), oxidase (-) with thick non-hemolytic colonies on BAP.

The biochem test's result naman revealed the following...

• Triple Sugar Iron (TSI) = A/A (lactose fermenter), gas production (+), and H2S (-)

• Sulfide Indole Motility (SIM) = motile (), indole (-), H2S (-)

• Simmon Citrate Agar (SCA) = citrate (+)

• and Lysine Iron Agar (LIA) = K/A lysine deamination (-)

If you noticed there's no sign on whether it is motile (+) or non motile (-). That's because I'm so unsure. Well, actually I was so sure it was motile when I left the lab this afternoon but when I got home, right now while I'm making my written report, I decided to look at pictures of Enterobacter cloacae and Klebsiella pneumonia online.

I stumbled upon a picture of K.pneumonia on BAP and its so similar to my unknown's growth on BAP.... which is unfortunate and is what made me hesitant because I was so sure it was E.cloacae because it's motile and K. pneumonia is non-motile. But now, looking back, I feel like it wasn't really motile on the SIM tube cause it wasn't totally hazy on the point of inoculation. Gosh...  To think that I even performed hanging-drop microscopic technique just to see the motility and because of a stupid picture online, I got confused. I hate this!!!

Clinical Bacteriology

Just wanted to put a record here of my progress in our final unknown in Clinical Bacteriology. We were given a bacterial suspension (the "unknown") yesterday and we inoculated it in Blood Agar Plates and Mac Conkey Agar media then incubated for 24 hours.

Here's what I came up with when we checked it out kanina...  As you can see on the pic below, it has gamma-hemolytic colonies which are very thick and aggregating... probably due to the slightly wet (really bad) BAP we all were using.

[caption id="attachment_127" align="aligncenter" width="300" caption="to be identified for Clinical Bacteriology"][/caption]

Don't ask me what happened to the one planted on the MAC... Halos di na makita yung streaking pattern dahil nag-swarm, again probably due to the wet medium plus sabi ni Sir Ems (our lab technician) baka na-contaminate daw. It has clear colonies sa gitna, then sa mga colonies sa gilid medyo nag-pink, so you cant tell anymore if it's a lactose fermenting bacteria or not. No worries naman cause we can determine that sa TSI.

So anyways, back to the BAP. I did Gram Staining as well this afternoon; twice actually. I had to make sure I really had a Gram (-) bacteria because it grew on MAC kahit papano. I think nakulangan ako ng decolorization the first time kaya may naiwang purple cells. Nung inulit ko, without pagmamadali and observing proper staining techniques, nawala naman ung mga false gram (+) cells and pure pink na silang lahat. If you observe the pic below, their actually rod shaped in... uhm... I'm not sure kasi may mga single, may magka-pair, may mga areas din na magkakadikit sila talaga so I cant tell wether the arrangement is single, in pairs, or in clusters. Sabi basta may nakita ka daw ilang humiwalay mag-isa na cells, single na daw yun. Oh well....

[caption id="attachment_129" align="aligncenter" width="300" caption="final unkown's gram stain"][/caption]

Anyways, I did the biochemical tests soon after staining. I did oxidase which gave a negative result (no purple end color), catalase positive (vigourous bubble formation), and the Enterobactariaceae tests namely TSI, SIM, SCA, and LIA; the results of which requires 24 hours of incubation so I'll get to that tomorrow.

So, after 2 days of action, the results for my final microbiology unknown are:

• Gram (-) bacilli with singular arrangement
• Gamma (non) - hemolytic bacteria
• Catalase (+)
• and Oxidase (-)

Will get to the rest of the biochem tests tomorrow and I'll see if I can make an interpretation by then. That'll be all for now. Ciao :D